UV-B Protective and Antioxidant Activities of Protein Hydrolysate From Sea Cucumber (Holothuria scabra) Using Enzymatic Hydrolysis

Sea cucumber is rich in protein that can be used to prepare a potential derived bioactive peptide for antioxidant and protective effect against UV-B induced skin cell damage. This study aimed optimize preparation of sea hydrolysate with both activities using three commercial enzymes response surface methodology (RSM) face-centered central composite design (face- centered CCD). Hydrolysis time concentration enzyme effects on the degree hydrolysis (DH), yield, hydrolysates were determined. The optimum conditions papain (SCP), alcalase (SCA), or flavourzyme (SCF) 3.6, 5.0, 4.1% (w/w protein), respectively, 360 min. resulting had DH 81-91%, yield 13-14%, IC 50 DPPH radical scavenging activity 0.3-4.1 mg/mL, FRAP 0.5-0.6 mmol FeSO 4 /mL, ABTS 1.3-1.6 mg/mL. was reported as HaCaT viability percentage after treatment. SCP, SCA, SCF showed 72.4, 74.5, 71.3% viability, respectively. 80% survival cells 0.21, 0.15 0.20 mg/mL SCA SCF, Thus, SCP selected isolation characterization. contained hydrophilic hydrophobic amino acids 42.4 57.6%, ultrafiltration Sephadex G-25 gel filtration chromatography done from SCP. Six peptides identified LC-MS/MS Leu-Val-Asn-Glu-Leu-Thr-Glu-Phe-Ala-Gln (1163 Da), Leu-Val-Asn-Glu-Val-Thr-Glu-Phe-Ala-Gln (1149 Phe-Val-Asp-Ser-Ser-Ala-Thr-Thr (826 Phe-Asn-Asp-Leu-Gly-Ala-Trp (821 Phe-Pro-Asp-Thr-Thr-Thr-Leu (793 Lys-Phe-Gly-Glu-Gly-Lys (664).